Topical Meeting in Biomacromolecular Dynamics by 15N NMR relaxation
Lund, April 18-19, 2023
April 18
12:00 Lunch
13:00 Brief theoretical background: molecular dynamics, stochastic processes, correlation function, spectral density function, model-free formalism, relaxation rate constants
15:00 Coffee
15:30 Exercises: interactive exploration of relationships between molecular dynamics and relaxation rate constants (molecular size, diffusion tensor anisotropy, S2, etc)
16:00 Pulse sequences: basic building blocks and experimental design, constraints, critical aspects, etc.
17:00 End day 1, check-in hotel, etc.
19:00 Dinner, discussions
April 19
09:00 Practical training on spectrometers (Bruker NEO, Topspin 4): setting up experiments, best practices, optimizing experiments (spectral parameters, recycle delays, pulse shapes, lengths and power levels, relaxation time points (length, number), etc.
10:00 Coffee
10:15 Practical training: data processing, evaluating intensities and baseplane noise, estimating uncertainties, etc.
12:00 Lunch
13:00 Practical training (using pre-recorded data sets): analyzing data in terms of rotational diffusion tensor, model-free analysis, etc.
15:00 Coffee
15:15 Practical training, continued
16:30 Wrap-up
17:00 End
Expected prior experience:
Basic knowledge of NMR spectroscopy. Being able to perform NMR experiments. Familiarity with Topspin (or VNMRJ).
Assigned reading prior to course:
Keeler J. “Understanding NMR spectroscopy”, 2nd Ed., ch 8.
Ferrage F. “Protein Dynamics by 15N Nuclear Magnetic Relaxation” in “Protein NMR Techniques”, ch 9, Methods in Molecular Biology, vol 831, doi:10.1007/978-1-61779-480-3_9