Topical Meeting in Biomacromolecular Dynamics by 15N NMR relaxation

Lund, April 18-19, 2023

April 18

12:00   Lunch

13:00  Brief theoretical background: molecular dynamics, stochastic processes, correlation function, spectral density function, model-free formalism, relaxation rate constants

15:00  Coffee

15:30  Exercises: interactive exploration of relationships between molecular dynamics and relaxation rate constants (molecular size, diffusion tensor anisotropy, S², etc)

16:00  Pulse sequences: basic building blocks and experimental design, constraints, critical aspects, etc.

17:00  End day 1, check-in hotel, etc.

19:00  Dinner, discussions

April 19

09:00  Practical training on spectrometers (Bruker NEO, Topspin 4): setting up experiments, best practices, optimizing experiments (spectral parameters, recycle delays, pulse shapes, lengths and power levels, relaxation time points (length, number), etc.

10:00   Coffee

10:15  Practical training: data processing, evaluating intensities and baseplane noise, estimating uncertainties, etc.

12:00   Lunch

13:00  Practical training (using pre-recorded data sets): analyzing data in terms of rotational diffusion tensor, model-free analysis, etc.

15:00  Coffee

15:15   Practical training, continued

16:30  Wrap-up

17:00  End

Expected prior experience:

Basic knowledge of NMR spectroscopy. Being able to perform NMR experiments. Familiarity with Topspin (or VNMRJ).

Assigned reading prior to course:

1. Keeler J. “Understanding NMR spectroscopy”, 2^nd Ed., ch 8.

2. Ferrage F. “Protein Dynamics by ¹⁵N Nuclear Magnetic Relaxation” in “Protein NMR Techniques”, ch 9, Methods in Molecular Biology, vol 831, doi:10.1007/978-1-61779-480-3_9