SwedNMR, Gothenburg University and Dr. Björn Burmann is hosting a Topical Meeting on Large Proteins/MethylNMR March 13th and 14th, 2023 at the Swedish NMR Centre Gothenburg. The scope of the meeting is to better understand both the practical and theoretical aspects of exploitation of the the favourable properties of side-chain methyl groups in proteins and beyond extending the size limit of solution NMR applications. The target group is SwedNMR staff scientists and experts as well as other NMR spectroscopists interested to learn more about this topic.
If you are interested to attend please contact Alexandra Ahlner or Björn Burmann (email@example.com, firstname.lastname@example.org )
11:30 – 12:15 Welcome, introduction and initial round table about the participants aim for the meeting
12:15 –13:30 Lunch Break
13:30 – 14:15 Part I: Methyl labelling Practical Aspects (Burmann)
How to label MALVIT methyl groups in coli
Different pre-cursors for different NMR applications
Alternative methyl labelling of cysteines, nucleic acids, methylation in general
14:15 – 15:00 Questions/Round Table
15:00 – 15:30 Coffee Break
15:30 – 16:30 Part II: NOESY-based approaches (Burmann)
Different NOESY based experiments for assignment and structural characterization
SOFAST based experiments vs. standard 3D/4D NOESYs
16:30 – 17:30 Questions/Round Table
18:00 – 19:00 Meeting Dinner
8:30 – 9:15 Automated NOESY assignment approaches (Burmann)
NOESY based automated assignment approaches (MAUS, MAGIC, methyl-Flya etc.)
Limitations of methyl-methyl NOEs
9:15 – 9:30 Questions/Round Table
9:30 – 10:00 Coffee Break
10:00 –11:00 Part III: COSY and other through-bond based assignment approaches (Orekhov?)
COSY type assignment approaches
HMBC-HMQC for stereo-selective valine and leucine assignment
11:00 – 11:15 Questions/Round Table
11:15 – 12.15 Part IVA: Methyl relaxation (Burmann or Orekhov)
Fast timescale (side-chain order parameter)
12:15 – 12:30 Questions/Round Table
12:30 – 13:30 Lunch Break
13:30 – 14:30 Part IVB: Methyl relaxation (Burmann or Orekhov)
14:30 –15:30 Round Table/Closing
Suggested Literature to read before the meeting
- Schütz, S. & Sprangers, R. Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems. Prog. Nucl. Magn. Reson. Spectrosc. 116, 56–84 (2020).
- Kerfah, R., Plevin, M. J., Sounier, R., Gans, P. & Boisbouvier, J. Methyl-specific isotopic labeling: a molecular tool box for solution NMR studies of large proteins. Curr. Opin. Struct. Biol. 32, 113–122 (2015).
- Boswell, Z. K. & Latham, M. P. Methyl-based NMR spectroscopy Methods for uncovering structural dynamics in large proteins and protein complexes. Biochemistry 58, 144–155 (2019).
- Clay, M. C., Saleh, T., Kamatham, S., Rossi, P. & Kalodimos, C. G. Progress toward automated methyl assignments for methyl-TROSY applications. Structure 30, 69–79 (2022).