Welcome to a workshop in Metabolomics at Linköping University 8-12^th of May, 2023. This workshop aims to give an overview of NMR based metabolomics for PhD-students and researchers that will apply this technique in their own research. Lectures and seminars/workshops on; overview of metabolomics, introduction to NMR, experimental design, biobanking of samples, workflow, sample preparation strategy, application of multivariate data analysis and clinical applications. The workshop contains practical laboratory exercises using NMR.

For information on how to participate in the workshop as a PhD course please visit:

https://old.liu.se/medfak/bkv/forskarutbildning/forskarutbildningskurser/karnmagnetisk-resonansspektroskopi-for-metaboliska-analyser?l=sv

To register for the workshop please fill in the following form:

https://forms.office.com/e/18DiJ5Krkf

For questions please contact Alexandra Ahlner (alexandra.ahlner@liu.se) or Bijar Ghafouri (bijar.ghafouri@liu.se)

Preliminary Schedule

Monday May 8^th

• Lunch

• Introduction to NMR-based Metabolomics

• NMR Theory

• Sample Preparation

• Introduction to group assignments

Tuesday May 9^th

 Guest Seminars:

• Hannah Röhnisch Eriksson, Methods for quantitative NMR-metabolomics and applications to identify disease risk biomarkers (Swedish Agricultural University, Uppsala)

• Rikard Landberg, Towards precision prevention with NMR-based metabolomics (Chalmers University of Technology)

• Anders Bay Nord, Standardized NMR-metabolomics for large sample sets (Göteborg University)

• Ilona Dudka, Tissue metabolomics by HR MAS NMR spectroscopy (Umeå University)

• Lunch

• Discussion with Guest Lecturers and Group assignments

Wednesday May 10^th

• Group discussion on group assignments

• NMR-based Metabolomic Study Workflow

• NMR Experiment Interpretation

• Experimental Design for a Metabolomic Study

• Lunch

• Group A (IFM, Campus Valla):

  • Spectrometer Set-up

  • Practical Data QC

• Group B (Campus US)

  • Group assignments and data analysis

 Thursday May 11^th

• Group discussion on group assignments and practical NMR metabolomics

• Metabolite Identification Strategies, Statistical Tools and Analytical Tools

• Lunch

• Group B (IFM, Campus Valla):

  • Spectrometer Set-up

  • Practical Data QC .

• Group A (Campus US)

  • Group assignments and data analysis

Friday May 12^th

• Group discussion on group assignments and practical NMR metabolomics

• Tips and tricks including trouble shooting 

• Lunch